连续流动分析仪与自动凯氏定氮仪测定小麦秸秆全氮含量之比较
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国家重点基础研究发展计划(973计划)项目(2013CB127402);中央级公益性科研院所专项资金资助项目(IARRP-2014-39)


Determination nitrogen in the Kjeldahl digests of plant samples by continuous flow analyzer in comparison with automated distillationtitration instrument
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    摘要:

    凯氏定氮法是测定植株全氮含量的经典方法,但费时费力。选择24个小麦秸秆样品,用浓H2SO4-H2O2消煮,分别利用连续流动分析仪与全自动凯氏定氮仪测定消煮液中氮含量,比较了两种方法测定结果,探讨利用连续流动分析仪测定植株样品全氮含量的可行性。结果表明:两种仪器测定的小麦秸秆中全氮含量无明显差异,彼此间呈显著线性相关,回归直线方程为Y(连续流动分析仪-N)=0.892X(凯氏蒸馏滴定-N)+0.753,相关系数r=0.942 1(n=24,P<0.01)。连续流动分析仪测定的回收率在96.6%~102.3%之间,对5个样品消煮液中氮浓度分别重复测定5次,相对标准偏差在5%以下。连续流动分析仪分析速度快,消耗试剂少,可用于大批量H2SO4-H2O2消煮的植株样品中全氮含量分析。研究结果为采用连续流动分析仪测定植株全氮含量提供了技术依据。

    Abstract:

    Kjeldahl distillationtitration has been used as a reference method for N determination in plants,but it is time consuming.This article aimed at establishing a time saving method for determining of plant N concentrations by continuous flow analyzer(CFA).Twenty four of wheat plant samples were selected and digested with H2SO4H2O2.N contents in the digests were determined by CFA and an automated Kjeldahl distillationtitration(AKDT)instrument,respectively.The test showed that there was no any significant difference of the plant N contents measured by the CFA and the AKDT method.A linear relationship best described the measured N values generated by both methods:CFA-N=0.892 AKDT-N +0.753.The Pearsons correlation coefficient was 0.942 1 with a significance level(n=24,P<0.01).The CFA method for plant N measurement had a high precision with relative standard deviation less than 5%.The standard recovery rate of feed samples with addition of(NH4)2SO4 was 96.6%~102.3%.It suggested that the CFA based on colorimetric detection of the pure ammonium chloride distillate(Berthelot reaction)could be used to determinate N in the Kjeldahl digests of plant samples.The CFA method was labor saving and could handle large numbers of samples.The human error in mixing and other operations was reduced to a great extent.

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温云杰,李桂花,黄金莉 ,刘云霞,高翔,汪洪*.连续流动分析仪与自动凯氏定氮仪测定小麦秸秆全氮含量之比较[J].中国土壤与肥料,2015,(6):146-151.

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  • 收稿日期:2014-11-30
  • 最后修改日期:2015-01-08
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  • 在线发布日期: 2015-12-17
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