In order to select the minimum chlorine content of fluecured tobacco in Henan，Yuyan 10，Yuyan 12 and Zhongyan 100 were used in the field experiment to study chlorine absorption and accumulation of different fluecured tobacco.The results indicated that chlorine content varied from 0.62% to 0.91%，0.54% to 0.82% and 0.43% to 0.67% for Yuyan 10，Yuyan 12 and Zhongyan 100 over the whole tobacco growth stages，respectively.The chlorine content appeared more than 08% in part of Yuyan 10 and Yuyan 12 leaves，while Zhongyan 100 met chlorine content in tobacco<0.8% requirement.30~75 d after tobacco transplanting was a critical period for chlorine accumulation in tobacco leaves，and chlorine accumulation ranked as Yuyan 10＞Yuyan 12＞Zhongyan 100.The chlorine distribution of the tobacco decreased in the order leaf(44.75%~77.35%)＞stem(12.22%~47.52%)＞root(1.66%~11.21%)Compared with other genotypes，Zhongyan 100 had more appropriate chemical components，and the output were 1.14 and 1.50 times for those of Yuyan 10 and Yuyan 12，respectively.Based on these results，Zhongyan 100 could be recommended as the most appropriate tobacco for chlorine reduction in experimental region.