In order to improve soil quality,reduce the excessive application of chemical fertilizer in the field of tobacco planting,and to improve the quality of tobacco leaf,biochar was prepared from tobacco stalk using high temperature and high pressure steam explosion technology.The effects of biochar and nitrogen fertilizer application on dry matter accumulation,chemical composition and sensory quality of fluecured tobacco were studied.Results showed：with N1（22.5 kg/hm2),N2（37.5 kg/hm2）and N3（52.5 kg/hm2）levels,the application of tobacco stalk biochar significantly increased the dry matter accumulation of fluecured tobacco up to 94% in the middle and late stage of growth.Under the condition of low nitrogen and medium nitrogen,2.4 t/hm2 tobacco stalk biochar was used to promote the growth of fluecured tobacco and improve the quality of tobacco leaf.