To promote cyclic utilization of the local agricultural organic waste resources,taking cow dung,chicken manure,mushroom residue as the main raw material,adding different microbial agents in composting and coordinating carbon and nitrogen ratio of 30∶1,the changing tendency of temperature,pH,total nitrogen,ammonium nitrogen,nitrate nitrogen and germination index in the different microbial agents composting process were investigated,and the nutrient contents of different treatments before and after composting were compared.The results showed that the initial composting temperatures wtih bacillus subtilis agents and EM agents were faster,fermentation base agents and quick decomposing agent were slower.Bacillus subtilis agents composting temperature substantially maintained at about 70 ℃ in hightemperature phase,the loss rate of nitrogen was the highest by 33.18% after composting,the germination index was the lowest,but EM agents composting temperature was 55～68 ℃ in hightemperature phase,the loss rate of nitrogen was the least by 10.73%,the germination index after composting 21 d was 51.55%,reaching basic maturity,composting 35 d up to 84.27%,reaching full maturity,composting maturity of EM agent was faster than other treatments.Therefore,using of cow dung and other organic waste hightemperature aerobic composting process,we need to choose the composting agents which contain a variety of microorganisms which are able to coordinate with each other and adapt to the effects of high temperature,and suitable amount of agents should be used to reduce nitrogen loss and improve the composting effect.