The tricalcium phosphatesolubilizing ability of phosphobacteria and their combinations was tested using laboratory shakeflask culture.The culture broth of W2+W3+W4 produced the highest available P content,which was 609.1 mg/L.Thus,W2+W3+W4 was selected as the optimal combination of phosphobacteria.Then the best combination strain culture conditions were optimized through the single factor test and orthogonal test.The results showed that W2+W3 +W4 had greatest culture effective phosphorus content with glucose as carbon source and ammonium sulfate as nitrogen source,the content was 609.1 mg/L and an increase of 18.4 times than the control.Orthogonal test showed that W2+W3+W4 the optimal culture conditions was 15 g/L glucose,0.67 g/L ammonium sulfate,culture medium initial pH 7,and 1% the inoculation quantity.After further experiments,the dissolve tricalcium phosphate content of W2+W3+W4 under the optimized was 664.29 mg/L,which was 55.19 mg/L(P< 0.05)more than that of normal fermentation broth.