The study explored the influence of longterm fertilization on soil pH,organic matter,and the contents of Zn in both soil and maize in the longterm fertilization experiment in Shenyang Agricultural University.The most important aim was to investigate the mechanism involved in the transportation of Zn in the soilplant system.The experiment was carried out since 1979.The maizemaizesoybean rotation system was used in the experiment. The treatments were N1P,N1PK,N1,N2,CK,M1N1P,M1N1PK,M1N1,M1N2,M1,M2N1P,M2N1PK,M2N1,M2N2 and M2.In these treatments,CK meant that no fertilizer was applied,N1 and N2 meant that 120 and 180 kg/hm2 nitrogen fertilizers were used respectively,and M1 and M2 meant that 13.5 and 27 t/hm2 organic fertilizers were used in soil.The soil samples were collected from 0～20 cm top soil at different fertilization plots at harvest in 2015. The pH,organic matter,as well as the contents of Zn in soil and in straw and grain of maize were determined.Compared to that in original soil in 1979,the present soil pH for the most of treatments significantly decreased.The pH decreased by 1～2 units at the chemical fertilizer treatments,decreased 0.5～0.8 units at the chemical fertilizer combined with organic fertilizer treatments,and slightly increased at the solely organic fertilizer treatments.The contents of organic matter,total and available Zn decreased at the no fertilizer treatment,and remained almost unchanged at the most of the chemical fertilizer treatments.However,at the organic fertilizer treatments,the contents of organic matter,total and available Zn,as well as the carbonated and organicbinding Zn significantly increased.Similarly with in soil,the content of Zn in maize was the lowest at the no fertilizer treatment,and were significantly high at the chemical fertilizer combined with organic fertilizer treatments.The pH was not negatively correlated with available Zn,while the organic matter was positively correlated with available Zn,total Zn in soil,and Zn in grain and straw.The longterm tillage and fertilization caused the significant decrease for the soil pH.The decrease of pH was induced mainly by the use of nitrogen fertilizers.The longterm no fertilization resulted in the significant decrease for Zn contents in soil and maize.While the longterm use of organic fertilizer markedly increased Zn contents in soil and maize.The organic fertilizer firstly increased the amount of organic matter which promoted the formation of organically bounded Zn and carbonate bounded Zn.pH had a weak influence on the Zn activity.