The objective of this paper was to investigate the effect of biological fertilizer on the growth，yield and quality of malting barley，and the malt quality with ‘Ganpi 6’as material.The results showed that there was no obvious effects on barley growth and development，including seedling stage，jointing stage，booting stage，heading stage when biological fertilizer was applied，but the mature period was delayed for 2 days compared with CK，and some traits including malting barley plant height，panicle，stem，leaf and sheath growth were promoted by the application of biological fertilizer;In the aspect of production，the application of biological fertilizer obviously improved the barley spike number，grains per panicle，and thousand grain weight，of which the most significant effect was on spike number，followed by grains per panicle，and the thousand grain weight was the minimum.Compared with the control，the application of biological fertilizer increased production by 3.4%，but the economic coefficient was decreased by 1.3% for absolute value and 2.6% for relative value.From the quality of raw wheat，the application of biological fertilizer promoted grain protein content，starch content,plumpness and 1 000 grain weight，and decreased blighted grain rate;In the aspect of malt quality，the application of biological fertilizer increased malt differential leaching rate，coarse powder，αleaching rate of amino nitrogen，soluble nitrogen content with varying degrees，and decreased saccharifying power，viscosity，thickness of powder，βglucan content，protein content with different degrees，but there was no effect on the time of saccharification and chroma.