It is of great importance to maintain high yield and high grain quality under reduced nitrogen supply towards a resourceeconomically and environmentfriendly agriculture model.The objective of this study is to study the effect of reduced nitrogen supply on the accumulation of zinc and iron in wheat,to provide reference for screening wheat germplasm with higher zinc and iron concentration on a large scale.Utilizing sand culture experiment in glasshouse,effects of four nitrogen supplied levels on agronomic traits and zinc,iron utilization were studied for four varieties(Ji mai 22,Shi 4185,Hatcher,Intrade).Ear number and aboveground biomass decreased following reduced nitrogen supply regardless of ability of tiller forming;zinc and iron concentration in grain decreased with reduced nitrogen supply;significant difference of grain zinc and iron concentration existed between different genotypes.Screening wheat germplasm with higher zinc,iron concentration and integrating such germplasm into breeding program is a feasible approach,which need to be carried out in future.