镁肥对缺镁葡萄叶片活性氧含量和保护酶活性的影响
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Effect of magnesium fertilizer on active oxygen content and protective enzyme activity of magnesium deficiency grape leaves
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    摘要:

    探讨大田条件下,补镁对缺镁葡萄叶片活性氧含量、保护酶活性和产量的影响,为葡萄科学施肥提供理论依据。采用裂区试验,主区设4个土施硫酸镁梯度,分别为0、112.5、150、187.5 kg/hm2,副区设3个叶面喷施硫酸镁浓度,分别是0%、0.2%、0.4%。结果发现:葡萄缺镁时,叶片超氧阴离子产生速率加快,过氧化氢含量增加,超氧化物歧化酶活性升高,但过氧化物酶和过氧化氢酶活性显著降低,叶片脯氨酸和丙二醛含量升高,生物膜受到伤害。通过土施和叶面喷施的方式适量补镁,葡萄叶片活性氧产生速率降低,同时保护酶过氧化物酶和过氧化氢酶活性增加,膜脂过氧化产物丙二醛含量降低,生物膜的受伤程度得到缓解,叶片衰老速率降低,葡萄产量提高,但当施入量过大对生物膜受伤程度的缓解效果降低,葡萄产量降低。土施150 kg/hm2同时叶面喷施0.4%硫酸镁肥时,叶片超氧阴离子产生速率低,过氧化氢含量少,膜质过氧化产物丙二醛含量低,葡萄产量高,是该土壤环境下的最佳补镁措施。

    Abstract:

    Under the condition of lack of magnesium,effects of magnesium supplementation on reactive oxygen content and protective enzyme activity of magnesium deficiency grape leaves were studied in order to provide reference basis for formulating rational fertilization technique of grape.A field experiment with a split plot design was conducted,four soil gradients of 0,112.5,150,187.5 kg/hm2 were set up in the main area,three concentrations of foliar spraying,which was 0%,0.2%,0.4%,respectively,were set up in the deputy district.The results showed that:when the grapes had magnesium deficiency,ultra oxygen anion producing rate was accelerated,hydrogen peroxide content and superoxide dismutase (SOD) activity were increased,peroxidase and catalase activity significantly were reduced,leaf proline and malondialdehyde content increased,biological membrane was injured.Moderate amount of magnesium application through soil or spraying on the leaf decreased the reactive oxygen production rate,and increased protective enzyme peroxidase and catalase activity,reduced the product of membrane lipid peroxide malondialdehyde content,alleviated the injury degree of biological membrane and leaf senescence,but when the amount was too large,the yield was decreased.With 150 kg/hm2 soil application and foliar spraying 0.4% magnesium sulfate fertilizer at the same time,the ultra oxygen anion producing rate,hydrogen peroxide content and membranous peroxide malondialdehyde content are all low,while the yield is high,which indicated it is the best magnesium supplement measure in this experiment condition.

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马晓丽,颜秋阳,刘雪峰,袁项成,向苹苇,杨梅*.镁肥对缺镁葡萄叶片活性氧含量和保护酶活性的影响[J].中国土壤与肥料,2018,(6):154-160.

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  • 在线发布日期: 2018-12-19
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