The influence of the composting mixture in different initial moisture content，C/N and straw crushing size on cattle manure aerobic composting were studied to find out the best ratio of parameters，and direct poultry farms and organic fertilizer manufacturers carry out reutilization of cattle manure and straw scientifically and effectively.Fresh cow manure and wheat straw were taken as raw materials in composting.On the basis of adding microbial culture，an experiment was conducted to verify the indicators of pile temperature and total nutrient（N，P2O5，K2O).The conclusion shows that:（1）The period of every groups fermentation temperature ≥45℃ were more than 14 d，which accords with the safety and hygiene requirements in NY/T 1168—2006 “Technical requirement for nonhazardous treatment of animal manure”；(2）When the crushed wheat straw was added in the fermented cow manure，the moisture content and C/N can be regulated，and the particle size and porosity of the pile are increased.The experimental results show that when the initial moisture content is about 60%，C/N is about 30∶1，and wheat straw size≤1 cm，it is the best condition to fermented manure.