Pulsed integrated amperometric ion chromatography was applied to determine monosaccharides in soil samples. CarboPACRTM PA10 column was used as the separation column,NaOH solution(12.5 mmol·L-1)was used as mobilephase,the Au and Ag/AgCl were used as working electrode and reference electrode respectively.Soil samples containedrhamnose,arabiasugar,galactose,glucose,mannose andxylose,fructose.The limit of quantification was between 0.4～ 4.3 μg·L-1.Values of recovery found by standard addition method were in the range of 97.8%～115.1%.The method is suitable for the determination of monosaccharides in soil samples.