This paper tried to isolate an efficient bacteria against Ralstonia solanacearumoftobacco，optimize its culturecondition，identify itsphylogeny，and provide the excellent strain resources for the production of tobacco bio -fertilizer production. R.solanacearumantagonist bacteria was screened by assessing inhibition zonesmethod，the culture medium andthe culture conditions of it was optimized by the single factor condition analysis，and the bacteria was identified by phenotypiccharacterization，physiological -biochemical and 16S rRNA gene sequence analysis. A R.solanacearum antagonist bacteria MT-002-B-7 wasscreened，its inhibition zone diameter reached 28.67 mm. The fermentation study showed that the optimal culture medium for MT-002-B-7 was 10%glucose，10% glutamicacid，culture volume10%(v/v)，initial pH7.0，inoculum size3%(v/v)，temperature35℃，shaker speed 150r/min，culture time 25 h. And the strain was identified as Pseudomonas aeruginosa. Bacteria MT-002-B-7 had good effect on the antagonism against tobacco bacterialwilt，it exhibiting broad application prospects in the tobacco bio-fertilizer production.