PCR-DGGE technique was used to study the changes of fungal community structure in different rhizospheresoils of rotation，continuous cropping flue -cured tobacco for 3 years and 6 years，so as to provide theoretical basis for thecontrol of tobacco continuous croppingobstacle.The results showed that for both the rotation and the continuous croppingfungi were the most dominant microorganism in the tobacco plant in the laterperiod.Continuous cropping significantly changed the fungal community structure of the rhizosphere soil of flue-curedtobacco，and the function was greater with thelonger continuous cropping period.DGGE band recovery sequence analysis and RDA species factor showed that long -termcontinuous cropping of soil fungal flora structurechanged，pathogensincreased，and tobacco leaf spotpathogens，Pythium，Mortierella，Alternaria，the genusSclerotia，Scenedesmophilus， Fusarium and Xylariomycetidae were closely related tosoil nutrient metabolism.Therefore，different cropping years have a great influence on the fungal community of flue -curedtobacco rhizospheresoil，and the change of fungal population structure after continuous cropping of flue -cured tobacco may be one of the main causes of flue-cured tobacco continuous cropping.