In thispaper，the soils of three different land use types(farmland，urban green space，unusedland)inLanzhou new district are studied，the activities of soil urease，catalase，invertase and soil organic matter and nutrientcontents aredetermined，also the effects of different land use types on soil organic matter and nutrient and enzymeactivities areanalyzed.The results of soil organic matter and nutrient determination show that the contents of organicmatter，total nitrogen and hydrolyzed nitrogen in surface soil of farmland are the highest，compared with farmlandthe contents in urban green space are14.7%，12.2%，13.0% lower respectively and the contents in unused land are57.7%，46.3%，26.2% lower respectively.The contents of organic matter，total nitrogen and hydrolytic nitrogenall show the trend of farmland>urban green land>unused land.However，the contents of available phosphorus andavailable potassium in the topsoil of urban green space are the highest，compared with urban green space，the contents infarmland are 14.2% and 18.4% lower respectively，the contents in unused land are 84.7% and 30.7% lower respectively.The contents of available phosphorus and available potassium all show thetrend:urban green space>farmland>unusedland.The results of soil enzyme activity determination show that the activities of urease in surface soil are as follows:farmland(1.15 mg ·g-1)>urban green land(0.82 mg ·g-1)>unused land(0.35 mg ·g-1)，the activities of catalase are asfollows:farmland(1.90 mL ·g-1)>urban green land(1.62 mL ·g-1)>unused land(0.79 mL ·g-1)，the activities of invertase areas follows:farmland(13.02 mg ·g-1)>urban green land(11.64 mg ·g-1)>unused land(8.61 mg ·g-1).The activities ofurease，catalase and invertase in the same depth vertical soil profile show the trend of farmland>urban green land>unusedland.The aforementioned three soil enzymes activities show a decreasing trend with the deepening of vertical soilprofile，and the value of the 0～ 20 cm layer is significantly higher than 40～ 60 cmlayer.In the urbanization process of Lanzhounewdistrict，the change of land use type has significant effects on soil organic matter and nutrient and enzymeactivity，and the effects are basically beneficial.