Phosphorus-soluble microorganisms play an important role in the transformation of insoluble inorganicphosphorus in soil.Whether the application of phosphorus fertilizer will affect the inoculation effect of phosphorus -solublemicroorganisms inproduction，and how to realize the rational application of phosphorus -soluble microorganism fertilizer andphosphorus fertilizer are worthy of further discussion.In this paper，corn，peanut and wheat were used as experimentalcrops.Under the condition of using phosphorus fertilizer，the effects of phosphorus -soluble strain Penicillium P8 on cropbiomass，phosphorus uptake and soil available phosphorus content werestudied，and the effects of phosphorus fertilizer on the inoculation effect of PenicilliumP8 were discussed.The results showed that the biomass of maize and peanut was not significantly increased by inoculating Penicilliumsp. at the same time in the first crop of potexperiment，compared with thesingle crop of phosphorusfertilizer，but the phosphorus uptake was promoted by inoculating Penicilliumsp.8.In the secondcrop of pot experiment， Penicilliumsp. 8 significantly increased the biomass and phosphorus uptake of maize and peanut， and Penicilliumsp. 8 had higher phosphorus uptake.The effective phosphorus content of the soil inoculated with PenicilliumP8 was higher than that of the soil withoutinoculation.In the pot experiment ofwheat，the yield of the soil inoculated with PenicilliumP8 increasedsignificantly.With the increase of P8dosage，the biomass of wheat increasedgradually，and the phosphorus uptake ability of wheat was effectively improved by the combination of Penicilliumand phosphorusfertilizer.The survival of PenicilliumP8 in soil was improved by using phosphorus fertilizer.The survival rate of Penicillium P8 in sterilized soil was higher than that in non-sterilized soil.