In order to find out the high yield cultivation techniques of taxus chinensis，taking taxus chinensis seedlings with consistent growth as test material，the effects of spraying different concentration of 5 -aminolevulinic acid(ALA)(0，10，20，40 and 60 mg·L -1)on photosynthetic characteristics and growth status of taxus chinensis seedlings were studied.The net photosynthesis(Pn)，stomatal conductance(Gs)，intercellular carbon dioxide concentration(Ci)，transpirationrate(Tr)of each Taxus chinensis seedlings were measured by Li -6400XT convenient photosynthetic system.The light response curve was simulated by Michaelis-Menten equation，and the growth indexes of Taxus chinensis seedlings were also measured.The results showed that:Compared with thecontrol group，spraying10，20，40，60 mg/L ALA increased the net photosynthetic rate，stomatal conductance，transpiration rateand growth status of taxus chinensis seedlings.The effect of ALA sprayed with 20 mg/L was the most significant.Compared with thecontrol group，spraying10，20，40，60 mg/L ALA also changed the fitting parameters of photo response curve of taxus chinensis seedlings，and it increased the maximum net photosynthetic rate，light saturation point，apparent quantum efficiency and reduced the light compensation point and dark respiration rate of taxus chinensisseedlings，and the effect of ALA sprayed with 20 mg/L was the mostsignificant.Inconclusion，in the range of experimentalconcentration，ALA of 20 mg/L was the most suitable concentration for promoting the growth of taxus chinensisseedlings.