The field experiment was conducted to study the reduction effect of microbial agents on cotton continuouscropping obstacles inXinjiang.The results showed that the application of the microbial agent had a significant inhibition oncotton verticillium wilt in continuous cropping，which can effectively reduce the incidence index and incidence rate whilereducing the disease.The control efficiency could reach about 50% and the yield was increased by9.64%~26.21%.Theapplication of microbial agents significantly increased the content of alkalinitrogen，available phosphorus and potassium by15.4%，36.8%，19.8% in continuous croppingsoil.The activities of soilurease，phosphatase，sucrase and catalase weresignificantly increased in microbialtreatment.It also reduced soil pH through metabolic activities such as enzymeactivity， increased the availability of phosphorus and potassium in soil and reduced the fixation of soil to new application of phosphorusand potassium fertilizer.The microbial agent significantly reduced the MDA content of cotton roots by 40.9% and increasedthe root activity by18.8%~68.8%，thereby reducing the self -toxic effect of continuous cropping on cotton and continuous cropping obstacles.