To improve soil colonization efficiency of growth promoting microbe and soil nutrient content，strains with high acid-tolerant and nutrient conversion efficiency were screened and immobilized on spherical carrier.Results showed one growth-promoting strain of genus Rhodotorulawas screened from tea plantingsoil，namedWYS2.The best immobilized agent was obtained when 1% sodium alginate + 2% agar as embedding material after 2% WYS2 cells were adsorbed by 0.25% activatedcarbon.After application of organic fertilizer，the soil colonization effect of immobilized agent was 293times higher than that of free cell suspension.Compared with the treatment without adding strain WYS2 and adding freeWYS2 cells suspension，in immobilized WYS microbial agent treatment，soil alkali -hydrolyzed nitrogen content，soil available potassium content and ammonium nitrogen content were increased by 23.4%～39.8%，18.1%～ 25.1% and 36.4%～45.1%，respectively.Inconclusion，the screened Rhodotorulasp.WYS2 can effectively colonize in tea plantingsoil after immobilizedapplication，and increase contents of soil available nutrients within a short period of time.