In order to investigate the effects of different substrate supply and substrate bag placement height on cucumbergrowth，yield，quality，nutrient accumulation and matrix nutrient utilization，six treatments were setup:three kinds for per plantsubstratesupply(5，7 and 9L)and two placement heights ofsubstratebags(ground placement and sinking 20cm)，using‘Bo Nai 526’cucumber was used as the test material.The results showed that the yield of cucumber was significantly improvedand the quality was improved obviously with the increase of substratesupply.When the substrate supply was 9L/plant，theyield of cucumber plants placed on the ground and sinking 20 cm of the substrate bag was thehighest，which were 3 443.17 and2 799.47 g/plant，respectively.At the same time，the cumulative amount of cucumberN，P and K also showed an increasingtrend with the increase of matrixsupply.When the substrate supply was 9 L/plant and substrate bag was placed on theground，theaccumulation ofN，P and K for cucumber was significantly higher than those of the other treatments during fullbearing，whichwere 2406.88，530.06 and 3 629.71 mg/plant，meanwhile，the P and K utilization rate of this treatment were also the highest，which were 56.59% and56.36%，respectively.Under the same substrate bag placement height，the soluble protein，vitaminC，reducing sugar and free amino acid of cucumber fruit with the substrate supply of 9 L/plant were thebest.The comprehensiveevaluation method was used to evaluate thegrowth，yield，quality and matrix nutrient utilization ofcucumber.The substratesupply was 9 L/plant and the ground cultivation had the highestscore.Under the cultivationmethod，the plantdevelopment，yieldand fruit quality weregood，and it could be applied to the actual production ofcucumber.