Ammonia-oxidizing bacteria(AOB)are of great importance as these nitrifiers oxidize NH 3 to NO₂ and reduce the loss of NH₃by volatilization in thermophilic phases during composting，making them ideal potential organisms for animal waste disposal. A thermophilic and micro-aerobic AOB was screened from the sample of high temperature composting. Data from this polyphasic taxonomy study suggested that strain BM62 should be classified as the type species of a new genus within the family Bacillaceae for which the name Aliibacillus thermotolerans is proposed.However，its ammonia oxidation capability declined significantly due to repeated iterations and other reasons. Aliibacillus thermotolerans BM62 can carry out ammonia oxidation in the micro-aerobic environment during the high-temperature stage of composting. This characteristic makes it highlytheoretically valuable and practically meaningful for nitrogen conservation in compost，therefore，it is necessary to studythe rejuvenation of the ammonia oxidizing ability of strain BM62.So，strain BM62 was selected as the researchobject，anddifferent nutrient factors(beef extract，peptone)were added to the source environment compost medium as the rejuvenation medium. The purpose of this paper was to investigate the effects of different rejuvenation medium on ammoxidation ability rejuvenation of the strain. The results showed that strain BM62 had the best rejuvenation effect in composting environmentrejuvenation medium without any exogenous organic matter. After rejuvenationculture，the ammoxidation activity of the strainBM62 was 34.7～ 36.5mg/L，which was equivalent with the ammoxidation capacity(35.0mg/L)before it was degraded.