To shorten the time of bacterial microorganism from screening to utilization in bio-fertilizerproduction，an easy way to determine its rhizosphere colonization ability in soil was necessary. Sensitive value dependent on the suppression of 21kinds of antibiotics to bacterial microorganism wascalculated，the rhizosphere colonization ability of bacterial microorganismwas tested by pot experiment，and the relations between sensitive value and rhizosphere colonization ability of bacterial microorganism was analyzed. The result showed a negative correlation between sensitive value and rhizosphere colonizationability，and rhizosphere colonization ability of bacterial microorganism was poor when sensitive value was more than 85. And it indicated that sensitive value could be used for determining the rhizosphere colonization ability of bacterial microorganism in soil.