In order to clarify the appropriate amount of nitrogen fertilizer for direct seeded rapeseed in Jianghuai area of AnhuiProvince，field experiments were set up with different amount of nitrogen fertilizer applied to study the effects of nitrogenapplication on the rape yield，nitrogen accumulation，economic benefit and nitrogen use efficiency.The results showedthat the yield and nitrogen accumulation of rapeseed increased significantly by the application of nitrogenfertilizer.When the amount of nitrogen application was higher than 240 kg/hm²，the rapeseed yield and nitrogen accumulation kept the sameyet.The highest economic benefit was 10 026 yuan/hm² when the amount of nitrogen fertilizer was 240 kg/hm².When the nitrogen fertilizer was 180 kg/hm²，the apparent utilization rate of rapeseed nitrogen fertilizer reached43.78%.When the application rate of nitrogen fertilizer was higher than 240 kg/hm²，the nitrogen harvest index and fertilizer utilization rate wereboth decreased.Under the same amount of nitrogenfertilizer，the rapeyield，nitrogen accumulation and nitrogen utilizationrate with urea applied separately were higher than that with urea applied at one time.Considering theyield，nutrientaccumulation，fertilizer utilization rate and economic benefit ofrapeseed，the optimal fertilizer application of direct seededrapeseed is 180～ 240 kg/hm²in the Jianghuai region of AnhuiProvince.