To clarify the changes of soil organic carbon pool and enzyme activity in greenhouse can provide scientific basis for the further study of soil organic carbon turnover mechanism and improvement of soil carbon storage in different plantingmodes.In thisstudy，the changes of soil organic carbon pool and enzyme activity in greenhouse in North China under threeplanting modes，i.e. organic(OP)，green(GP)and conventional(CP)planting modes，were studied by 16 yearsexperiments and quantitative assessment of soil quality wasconducted.The results showed that OP significantly increasedthe vegetables yield and soil organic carbon poolcapacity，improved soil structure，increased water content and nutrientcontent，and enhanced soil fertility and buffering performance.Compared with CP，OP increased the vegetables yield ofspring and autumn by 62.28% and22.02%，soil organic carbon by105.53%，labile organic carbon fraction increased by109.30%～230.27%，humic carbon fraction increased by 91.61%～128.37%，soil carbon storage increased by51.14%，respectively.The effects of GP was second to OP.The activity ofcatalase，urease and cellulase increased significantlyinOP，which increased by48.51%，68.39% and109.72%，respectively，but sucrose did not change significantly.According to the principal component analysis，OP>GP>CP can be known from the comprehensive score.Therefore，organic planting is superior to green and conventional cultivation in improving soilstructure，increasing soil nutrient contentand transformation efficiency，and increasing soil carbon sequestration.It is indicated that organic cultivation has asignificant effect on improving the content of organic carbon composition，carbon storage and enzyme activity in greenhousein NorthChina，and is the key measure to increase vegetable yield and soilfertility，improve soil structure and increase soilcarbonsequestration.