In order to explore the synergistic effect of amino acid as fertilizer synergist in the growth ofrice，a pot experiment was conducted to investigate the influence of the application of compound amino acid solution combined with common urea onthe growth of rice seedlings and soil nutrients. The results showedthat，compared with nofertilization(CK1)，rice seedlingheight，chlorophyllcontent(SPAD)，root growthindex，shoot dry weight and NPK cumulative uptake were increasedsignificantly with the extension of transplanting time by the application of 5% compound amino acidsynergist(A). Amongwhich，the plant height increased by 11.1%～18.1%，the dry weight of aboveground increased by 9.03%～24.00%，and the cumulative absorption ofN，P，K increased by 32.3%～57.0%，50.7%～74.0%，and 19.0%～40.5%，respectively. Compared with singleurea(U)application，the plant height and root growth index of rice seedlings increasedsignificantly in the early transplantingstage，the SPAD increased significantly in the late transplantingstage，and the dry weight of aboveground and the cumulative uptake of NPK increased with the transplanting time by the combined applicationof 5% compound amino acid synergist with commonurea(AU). Under the same nitrogen conditions，the AU treatmentimproved the nitrogen use efficiency(8.24%～31.50%)compared with the U treatment(6.08%～28.10%). Inaddition，for AU treatment，the total nitrogen content in rhizosphere soil decreased from 0.93 g/kg to 0.91 g/kg with the increase oftransplantingtime，which was lower than that of U treatment. And the contents of total nitrogen and available phosphorus in rhizosphere soil were higher than that in non-rhizosphere soil. These indicate that the addition of compound amino acid can promote the conversion of total nitrogen and available phosphorus from non-rhizosphere soil to the rhizospheresoil，andmaintain the nitrogen content in the rhizosphere soil at a high level. Insummary，the application of compound amino acid cansignificantly promote the growth of riceseedlings，and the combination of compound amino acid with common urea can alsoimprove the nitrogen absorption andutilization，and conducive to the accumulation of soil total nitrogen.