Using the soil samples with pH from 4.5 to 8.0 as the analysis objects，a method for determination of soil available sulfur content by ion chromatography was established.Soil available sulfur was first extracted by 0.01∶0.01(mol/L)CaCl2-KH2PO4 extractant，then analyzed by ion chromatography and quantified by external standard method.The results showed that the ion chromatography selected effectively separated soil available sulfur from interfering components.The quantitative method had good linearity，with the correlation coefficient r of 0.9997，the precision RSD(n=6) less than 5%，and the recovery rates of 88%～105% for the three concentration addition levels.This method was used to determine the available sulfur content in 4 soil quality control samples，and the results were consistent with the marked values.The analytical method for determination of soil available sulfur content established in this study is simple in pretreatment，accurate in results and good in reproducibility，and this has popularization and application value.