Rhizosphere soils of crop in the Yellow River Delta were collected to isolate high-efficiency phosphate-solubilizing bacteria to obtain microbes for bio-fertilizer production for saline-alkali soil. PVK solid medium was used to screen phosphorus-soluble bacteria by transparent circle method；16S rDNA sequence analysis combined with morphological characteristics were used to identify strains；LB medium containing different NaCl concentrations were used to determine the salt tolerance of the bacteria；liquid culture experiment was used to determine the ability of dissolving insoluble phosphorus sources. The phosphorus solubilizing efficiency of the strain was investigated in saline-alkail soil. A total of 27 phosphate-solubilizing bacteria were isolated and B19 identified as Pantoea cypripedii，was selected for further study. Pantoea.cypripedii B19 grows well at 0%～4% NaCl concentration and can tolerate salt concentration up to 6%. B19 had good phosphate-solubilizing capacity. When cultured on PVK solid medium at 30℃ for 3 days，the diameter of the solubilizing halo zone(D)was 18 mm，which was 3.17 times of the diameter of the colony(d)；B19 could effectively dissolve Ca3(PO4)2，AlPO4，FePO4 and phosphate rock powder，and released 230.2，72.1，153.2 and 28.5 mg/L phosphorous in the PVK liquid medium，respectively. The results of soil culture experiments showed that strain B19 significantly increased the level of available phosphorus by 36.2% in saline-alkali soil after 10 days. The results of pot experiment showed that the B19 had obvious promoting effect on the growth of wheat seedlings，which indicates that B19 is a promising candidate to develop bio-fertilizer suitable for saline-alkali soils.