To understand the long-term organic manure affecting soil nutrients balanced supplement in the plastic-shed vegetable soils converted from rice-wheat rotation，the organic plastic-shed greenhouse vegetable farmland was selected as the main study area in Nanjing，Jiangsu province.Soil samples were collected from the tilled layer(0～15 cm)and plough pan layer(15～30 cm)under 13 and 18 years organic vegetable plots(plastic-shed and open-air patterns)converted from rice-wheat rotation with long-term organic manure application.The result demonstrated that long-term organic manure significantly improved the soil dissolved organic carbon(DOC)，active organic carbon(AOC)，carbon pool index(CPI)，and carbon pool management index(CPMI)of plastic-shed greenhouse vegetable fields converted from rice-wheat rotation，except for carbon pool activity index(AI).However，these soil carbon properties showed a significant decrease later with the cultivation lengthening.There was non-significant difference in soil microbial biomass carbon(MBC)among these treatments.The plastic-shed greenhouse organic fields had higher soil NPK accumulation，C/N，C/P and N/P ratios compared with that of open-air organic vegetable fields.The soil C/N，C/P and N/P ratios of 18-year plastic-shed greenhouse organic vegetable fields was 7.32%，23.22% and 18.57% higher than that of open-air organic vegetable soils in the surface layer，respectively，but not in the plough pan layer.There was various correlation strength between soil different C factions and soil nutrients ecological stoichiometric parameters.In that，the C/N was strongly affected by soil C quantity，soil N/P was more influenced by soil organic C factions and CPI，and the soil C/P was affected by soil organic C quantity，quality and CPMI at the same time.To sum up，this study revealed that there is a significant improvement of long-term organic manure on soil nutrients accumulation，but the improvement effect is limited by time.It’s not a good choice to rely on organic fertilization to balance soil nutrients distribution.