枯草芽孢杆菌KD03液态菌剂的保存方法研究
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(1.河北科技大学食品与生物学院,河北 石家庄 050018;2.河北省发酵技术创新中心,河北 石家庄 050018)

作者简介:

赵国群(1963-),教授,博士,研究方向为工业微生物技术。E-mail:gqzhao18@126.com。同时为通讯作者。

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基金项目:河北省科技计划项目(19226920D)。


Study on storage of liquid microbial inoculant of Bacillus subtilis KD03 at normal temperature
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(1.College of Food Science and Biology,Hebei University of Science and Technology,Shijiazhuang Hebei 050018;2.Fermentation Engineering Center of Hebei Province,Shijiazhuang Hebei 050018)

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    摘要:

    保质期短是限制液态微生物菌剂在农业生产上广泛应用的主要因素。枯草芽孢杆菌KD03是一株具有良好抗黄瓜枯萎病的生防菌。为了提高KD03液态菌剂的菌体存活率、延长保质期,采用单因素试验研究了添加防腐剂、碳氮源、醇类物质以及菌剂pH值对菌体存活率的影响,在此基础上采用正交试验设计优化菌剂的保存条件。试验结果表明:KD03液态菌剂中添加防腐剂(山梨酸钾和苯甲酸钠)、碳源(葡萄糖)、氮源(酵母浸粉)、醇类物质(甘油和聚乙二醇)均可显著提高KD03的存活率。菌剂pH值为7.0或为接近7.0的弱酸性时,KD03菌体存活率高。KD03液态菌剂的最佳保存条件为:山梨酸钾1.0 g/L、甘油0.5 g/L、葡萄糖3.0 g/L,菌剂pH值自然(pH值6.7)。在此条件下,KD03液态菌剂室温保存6个月后,菌剂活菌数为3.98×109 CFU/mL,菌体存活率为82.2%。

    Abstract:

    Liquid microbial inoculant has a short shelf life,which is one of main factors that limits its use in agriculture. Bacillus subtilis KD03 has a perfect resistance against Fusarium wilt of cucumber.In order to improve the cell survival of B. subtilis KD03 and increase the shelf life,the effects of preservatives,carbon source,nitrogen source,alcohols and pH value on the cell survivals were investigated using single factor experiments.On this basis,storage conditions of KD03 inoculant were optimized by using orthogonal tests.The result showed that addition of preservatives(potassium sorbate and sodium benzoate),carbon source(glucose),nitrogen source(yeast extract)and alcohols(glycerol and polyethylene glycol)could significantly reduce the cell death and improve the cell survival of B. subtilis KD03.When the pH value of KD03 microbial inoculant was 7.0 or close to 7.0,the cell survival of B. subtilis KD03 was the highest.The best storage condition of KD03 inoculant at room temperature was at 1.0 g/L potassium sorbate,0.5 g/L glycerol,3.0 g/L glucose and the pH value of inoculum is natural(pH value 6.7).KD03 microbial inoculant was stored under the obtained best storage condition at room temperature for six months,the viable cell number of KD03 microbial inoculant was 3.98×109 CFU/mL,and the cell survival was 82.2%.

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赵国群,胡伟,白敬.枯草芽孢杆菌KD03液态菌剂的保存方法研究[J].中国土壤与肥料,2022,(9):203-208.

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  • 收稿日期:2021-07-31
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  • 录用日期:2021-09-10
  • 在线发布日期: 2022-11-22
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