There is still a lack of corresponding standard methods for the determination of nickel in fertilizers， using flame atomic absorption spectrometry(FAAS)，inductively coupled plasma emission spectrometry(ICP-OES)，and inductively coupled plasma mass spectrometry(ICP-MS)to determine the nickel content in fertilizers.In the experiment，four types of fertilizer samples，water-soluble fertilizer，compound fertilizer，organic fertilizer，and soil conditioner were selected，and the same pre-treatment method of aqua regia digestion was adopted，and the digestion solution was tested separately.The test results show that the detection limits of the three methods are:FAAS>ICP-OES>ICP-MS，which were 0.11 mg/L，0.02 mg/L and 3.94 μg/L，respectively，the relative standard deviation(RSD，n=6)of the determination results is between 1.59%～13.59%，and the recovery rate is 80%～118%.Correlation analysis results show that the correlation coefficient between the data measured by FAAS and ICP-OES is 0.9678，the correlation coefficient between the data measured by ICP-OES and ICP-MS is 0.9801，and the correlation coefficient between the data measured by FAAS and ICP-MS is 0.9649，which is a linear relationship.The test results show that there is little difference in the determination results of the three methods.The method for the determination of nickel in fertilizers was established through the research.The fertilizer samples were digested with aqua regia-electric hot plate.The content of nickel in the liquid can be determined by FAAS，ICP-OES and ICP-MS.The laboratoy can choose to use different detection methods according to the actual situation.