By means of pot,field minispot experiments and analysis indoor,the effects of soybean specific fertilizer on soil enzymes activity and available nutrients were investigated in rotation, soybean continuous cropping system,respectively. The results showed the soil enzymes activity and available nutrients in soybean continuous cropping could approach or higher than those of conventional fertilizeration after applying soybean specific fertilizer,which indicated soybean specific fertilizer could modulate the deteriorating soil enzymes and nutrients ecological surrondings ,therefore it could overcome the soybean drop in production caused by continuous cropping.