The effects of AM fungi on the quality of tobacco leaf in pot culture under five phosphrate levels were investigated by inoculation two AM fungi (Glomus mosseae and G.caledonium). The results showed that mycorrhizal infection rate could be promoted by inoculation.The quality of tobacco was also significantly affected.Two AM fungi increased the content of total sugar,nicotine,P,K and the ratio of general sugar and protein of tobacco leaf;while decreased the content of Fe, Zn and the ratio of nitrogen and alkali ,as well as the ratio of sugar and alkali.But there were some different effects between two fungi.It seemed that existed favoritest associational relation among fungi, hoster and soil. The co-effect of the inoculated and P_2O_5 0.056 g/kg on the content of chemical compositions were as same as the no inoculated and P_2O_5 0.112 g/kg.Therefore AM fungi can gain better quality of tobacco leaf in low phosphrate level.