The effect of potash and organic manure application on the content of 17 amino acids, crude protein and raw starch in wheat grain was studied based on a long term fertilizer trial located in Yutian County, Hebei Province. The content of aspartate, methionine, tyrosine and lysine in wheat grain was obviously increased by the application of potash and the combination of potash and organic manure. The content of methionine, lysine and proline was significantly increased by the application of organic manure . The extent of increase was all above 10%. The application of potash and (or) organic manure also increased wheat yield, total content of amino acid and crude protein, decreased the content of raw starch in wheat grain, however.