Effects of 20 years continuous long-term application of organic manure,chemical fertilizer and organic-chemical combined fertilizer on the results of soil nutrients variation were studied at Yucheng experiment area of Shandong Province.The experimental results indicated that at equal fertilization condition years after years,fertilizing organic manure not only had markedness fuctions at increasing soil's organic nutritions and improving quality of soil organic matter,but also could increase soil availabale nutrients more than chemical fertilizer.