In order to investigate the effect of phosphate bacteria on the validity of insoluble phosphates in fertilizer and soil, this paper used the methods of potted plant in soil, put nitrogen- fixing bacteria, phosphate bacteria, silicate bacteria and so on beneficial microorganism into organic fertilizer and then mixed it to the phosphate rock to use. The results showed that, in four program, the blank program; the phosphate rock; contain phosphate bacteria of bio - organic fertilizer; no phosphate bacteria of bio - organic fertilizer, the application of bio - organic fertilizer with addition of phosphate bacteria could obvious increase the biomass of maize, improve its biology characteristics including leaf number and plant height, enhance the NPK accumulation in plant. The better preference was showed in soils taken from Huizhou of Guangdong province. Also it could increase soluble phosphorus content in soil.