Through 5 years localization experiment, resoult indicated that for long- term organic inorganic fertilizer matches fertilizes grain fertility crop rotation , the interplanting matches fertilizes could enhance the yellow soil of type yellow soil, the yellow latefite soil of type red soil and moist soil of type submergie southern paddy soil organic matter, the humus and the active humus content, humic acid and furey acid also correspondingly increased, but by the grain fertility crop rotation, the interplanting matches fertilizes cultivated the fertility effect to be best. The grain fertility crop rotation, the interplanting match Hu which fertilizes also could enhance the soil H/R ratio. Each processing influence not only limited to the soil topsoil, but effect was remarkable by the 0 - 50 cm soil layer.