T_(42)(Trichoderma sp.) and Bs-6(Bacillus subtilis) had strong antifungal activity to FUS3(Fusarium oxysporium).The field experience results showed the mixed animacules could increase the length and diameter of watermelon stem and quicken the reproductive grow of replant watermelon.At the same time,economic yield and total yield were increased by 50.9% and 66.7%,respectively.The effect of the mixed animacules against watermelon replant disease was 75%.