Crude oil degrading bacteria which could utilige hydrocarbon compound as sole carbon source were isolated from ShengLi Oil Field. Among these bacteria, 31 strains could degrade hexadecane, 28 strains could degrade cyclohexane, 3 strains could degrade aromatic hydrocarbon and 24 stains produced biosurfactants; Oil degrading effect of bacterial community - C9 composed of strain SL- 51, SL- 84, SL- 133 and SL- 163 reached 55.5% in 5 days with oil concentration 0.5% ; Gas chromatography analysis proved that C9 degraded saturated hydrocarbon and aromatic hydrocarbon effectively; Stain of SL- 51, SL- 163 were identified as Rhodococcus spp., strain of SL- 84 and SL- 133 were identified as Ochrobactnan sp. and Pseudomonas sp. respectively.