The results of experiment indicated that the amount of bacteria,fungi and actinomyce of soil was increased,the activity of sucrase,urease,and Catalase of soil was increased too,promoted the growing of tobacco plants and economic characteristics of flue-cured tobacco after applying rapeseeds cake in soil.The amount of microbes of paddy soil was more than that of dry soil,its enzymes activity was also higher than that of dry soil.The tobacco plants grew well in paddy soil and its economic characteristics was better than that of dry soil.For tobacco leaf of paddy soil,its content of nitrogenous compounds was high,and the balance of chemical components was not ideal.The smoking quality of tobacco leaf of dry soil applied with rapeseeds cake was best,but it was worst for the dry soil applied no rapeseeds cake.