Pot experiment was carried out to study the effect of biogas fertilizer on the yield and quality of the lettuce in soilless culture.In experiment,the mixture of turf and perlite was used as substrate of culture. The experiment was designed to the biogas fertilizer group,the chemical fertilizer and the control group.Each fertilizer group included three fertilization levels,and the content of nitrogen,phosphorus and potassium biogas fertilizer was equal to chemical fertilizer under the same fertilization level.The experimental results showed that 100,200,300 g of biogas fertilizer was respectively fertilized in 2 L substrate could respectively make the yield of lettuce increases 58.55%,108.08% and 99.12%,the reductive sugar increases 11.59%,9.87% and 74.48%,the total sugar increases 4.31%,65.22% and 72.56%,Vc increases 106.67%,95.24% and 47.62%,the nitrate decreases 85.37%,82.63% and 87.61%,compared with fertilizing chemical fertilizer.So biogas fertilizer was fertilized in soilless culture not only increases the yield of vegetable but also remarkably improved its quality,and it was very important for developing innocuous and green agriculture.