For guiding for the fertilizer application,the effects of copper and nitrogen fertilizer cooperation application on grain yield and protein content of winter wheat through field experiment in Meadow soil in middle Henan Province was studied.The results showed that N fertilizer affected the yield and protein yield extremely,and had no remarkable effects on protein content.Cu fertilizer and the interaction of Cu and N had extremely remarkable effects on the grain yield and protein yield,and affected protein content remarkably. Under the conditions of this experiment,the N1Cu2 treatment was the most appropriate for grain yield and protein content and protein yield.