The results showed that CO2 gas by biological fermentation of organic waste in the greenhouses could be produced in a longer time at a high stability level，thereby contributing to strawberry plant growth and development，to improve the yield and quality of strawberry.After application of the CO2 fertilization，the quality of strawberry fruit was improved，the contents of vitamin C，soluble solids and soluble sugar were increased by 11.25 mg?100 g -1 ，1.0 % and 2.06 %，re- spectively，while organic acid and nitrate content were decreased by 0.10 % and 126.45μg?g -1 .Average single fruit mass was increased by 14.32 g，about 67 %.The yield was increased significantly by 29.9% per hectare.