Long-term fertilization trial was conducted to study the effect of various fertilizer application on organic matter of the black soil.The results showed that the longterm effects of fertilization on soil organic matter were different,dealing with the application of organic and inorganic fertilizer>organic fertilizer>inorganic fertilizer treatment>no fertilizer treatment.The longterm fertilization also affected the organic matter of deeper soil.The role of organic manure was particularly evident,and effects of fertilization on soil organic matter content showed a downward trend with the deepening of soil.Organic fertilizer on organic matter of different densities content increased in the most effective,while the application of inorganic fertilizer reduced organic matter content.