In order to investigate the effects of different fertilization with optimum chemical fertilizers，single organic manure，combination of organic manure and chemical fertilizers and conventional fertilization on paddy rice and spring wheat yield，N utilization and soil fertility in a paddyupland crops rotation system，a field experiment was conducted from 2008 to 2009.The results indicated that fertilization increased the grain and straw yields of paddy rice and spring wheat in contrast to CK(no any fertilizers);And at the same level of nutrients supplying，the highest and secondary yield increment were in treatment optimum chemical fertilizers and combination of organic manure and chemical fertilizers，respectively.The total shoot N uptake of paddy rice and spring wheat were significantly different from fertilization treatments.Apparent N recovery in paddy rice season was ranged from 104% to 370%，and which in spring wheat season ranged from 117% to 359%.Application of organic manure was in favor of increasing the soil organic matter，total N，soil available nitrogen，OlsenP，available K and C/N ratio.Soil alternate dryingwetting was also propitious to enhance the availability of soil nutrients under paddyupland rotation condition.Considering of crops yield increasing，apparent N recovery and soil fertility，combined application of organic manure and chemical fertilizers was the optimal fertilization practice in the paddyupland crops rotation system in this experiment.