To study the relationship between soil microbial quantity,enzyme activity and soil fertility in hot pepper greenhouse soils of different continuous cropping years and the adjacent open field of wheat-pepper rotation(as the reference).The results showed that the nutrient contents in the soils of continuous cropping years were higher than CK and pH was significant lower than CK.Soil bacterial,actinomyces quantity with the cropping years increasing increased at early period and then decreased and the values were higher than CK,and fungal quantity increased.The soil enzyme activity were significantly higher than CK.Soil bacterial quantity and organic matter,total P had significant correlation level，Fungi quantity and soil nutrient had no significant correlation.Actinomycetes quantity,catalase,Saccharase,urease activity and pH value were significant negative correlation level,and nutrient were significant correlation.Soil total P,total N,pH value great influence microbial quantity.Soil total N,pH value great influence soil enzyme activity.