Chlorimuron ethyl is a long residual herbicide.Soil contaminated with chlorimuron ethyl affect soil fertility and crop growth.In this paper,21 chlorimuron ethyl degrading bacterial strains were isolated.In pure culture,0.75%~80.77% of the 50 mg/L chlorimuron ethyl was decomposed after 7 days incubation.Results of 16S rDNA sequencing and phylogeny analysis showed that 21 chlorimuron ethyl degrading bacterial strains belong to Enterobacter,Pseudomonas,Citrobacter,Stenotrophomonas,Achromobacter,Brevibacterium,Shigella respectly.