The study was carried out to investigate the changes of soil enzyme activities,soil nutuient as well as their relationship in meadow alkaline soil under the treatment of organic manure application in different years,based on the Northeast Agricultural University long term experiment station of improving saline alkali soil.The results indicated that long term application of organic manure could significantly increase urease,invertase and neutral phosphatase activities in meadow alkaline soil,however it had little effect on catalase activity.The contents of organic matter,total N,P and available N,P in meadow alkaline soil were significantly increased,and the soil pH was significantly decreased after long term application of organic manure.Meadow alkaline soil fertility had been improved significantly after 5 years amount of fertilizing.The results of correlation analysis showed that the activities of soil urease,invertase and neutral phosphatase were positive or extremely positive corrected with organic matter,total N,P and available N,P,but there was no positively correlation between catalase activity and soil nutrient content.