The effect of continuous and multiple cropping of tobacco rice on soil available medium & trace elements and the corresponding elements in tobacco leaves was carried out in tobacco field.The results indicated that during 0~23 years of continuous and multiple tobacco rice cropping,the sulfur content in soil and tobacco leaf increased gradually;the soil available nutrients of other 9 kinds’ medium & trace elements showed different degree’ downward trend after 10~15 years of continuous and multiple tobacco rice cropping,meanwhile these corresponding 9 kinds of nutrient elements in tobacco leaves showed a downward trend directly or after 10 years of continuous and multiple cropping.The above changes were disadvantaged to the formation of good quality of flue cured tobacco.The correlation between soil available boron,molybdenum,sulfur,calcium and magnesium and the corresponding elements in flue cured tobacco leaves very significantly or significantly positive,other elements had no significant correlation.