In this paper，two oilseed rape varieties named X-13 and X-29 were used as plant materials for sand culture with the different nitrogen (N) application levels to study the different effects of N application levels on accumulation amount of nitrate，nitrate reductase (NR)activity，absorption amount of N，seed yield and N efficiency in the two oilseed rape varieties.The results showed that compared with X-29，the nitrate N accumulation is higher，reutilization ratio of nitrate，the activity of NR in leaf，N accumulation，amount and seed yield are increased in the X-13 treatment.And the difference between the two varieties is significant.But the increasing of nitrogen application level，nitrate accumulation amount，reutilization ratio of nitrate，and the activity of NR in leaf increased and the N efficiency decreased in both oilseed rape varieties.