In this paper field experiments were adopted to study the effects of applying chemical fertilizer combined with different organic matters on biomass accumulation，nitrogen uptake and nitrogen distribution of flue cured tobacco.The results showed that the distribution of nitrogen in the tobacco were leaf> stem> root，and the nitrogen uptake of upper leaves account for 20%~35% of the total nitrogen uptake，which was the highest N proportion in the leaves.In the treatment of the combination of chemical fertilizer and fowl dung，the accumulation of dry matter were 7.58% and 17.23% higher on the resettling stage and mature stage respectively than the treatment of 100% inorganic nitrogen applied，nitrogen uptake of upper leaves and middle leaves on the full ripe stage were respectively 137.99% and 40.7% higher than that of the 100% inorganic nitrogen applied，and all of the differences were significantly.Combined application of chemical fertilizer and fowl dung on the resettling stage and mature stage would lead to the thicker leaves and the delay of maturity than treatment of inorganic nitrogen.For the treatments of combination application of chemical fertilizer and rapeseed cake or maize straw，biomass accumulation on the resettling stage and mature stage were respectively 1.64%~5.11% and 3.99%~1.23% lower than that in the treatment of inorganic nitrogen.The nitrogen uptake and distribution in these two treatments were similar with that in the treatment of the local recommended N fertilizer amount，and could well balance the relationship of nitrogen supply and distribution in tobacco.