The effect of water soluble fertilizers on spinach was investigated.The water soluble fertilizer 1 (WSF1) was made by Burdock extracts and Burdock Oligosaccharide(BO).Water soluble fertilizer 2(WSF2)was made by Burdock extracts and Oligoehitosan(OE).Pot experiment was conducted to study the effects of purchased foliar fertilizer(PFF)，WSF1,WSF2,BO and OE on the growth,quality,physiology index,leaf and leaf stalk microscopic structure of spinach.The results showed that:Compared with those of CK treatment,the spinach fresh weights of treatments of PFF,WSF1,WSF2, BO and OE were increased by 870％ to 1677%,the spinach chlorophyll content were increased by 5.18% to 29.60％,nitrate content were decreased by 10.82% to 60.04%,oxalic acid content were decreased by 11.99% to 67.61％,malondialdehyde (MDA) content were decreased by 11.94% to 30.91%,and generation rate of·O-2were decreased by 43.58% to 72.99%.Compared with that of PFF treatment,the spinach fresh weights of WSF1 treatment was increased by 4.41％,nitrate content of WSF1 and WSF2 treatment were decreased by 49.03% and 42.80% respectively,and oxalic acid content were decreased by 11.32% and 52.2％ respectively,MDA content of WSF1 treatment was decreased by 4.09%,and generation rate of ·O-2of WSF1 treatment was decreased by 6.26%.In short,spraying water soluble fertilizer,Burdock Oligosaccharide and Oligoehitosan can increase spinach biomass and chlorophyll content of leaves,decrease nitrate and oxalate content,thereby improve the quality of spinach.MDA content and generation rate of·O-2were decreased at the same time,this suggested that antioxidant capacity was improved,cell membrane was complete and spinach leaf cells arranged closely,regularly and the epidermal arranged orderly after treated with BO and OE,considering all the properties of spinach,WSF1 was the best treatment in this experiment.